Histone H4(mono methyl K20) Recombinant Mouse Monoclonal Antibody

货号：

AWA06035

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 11 kDa
Observed MW: 15 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1.154mg/ml

Other Names:

dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652; H4K20(ME1); H4K20me1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF 1:50-1:200

Immunogen
Information

Gene Name:

H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16

Protein Name:

Histone H4

Gene ID:

121504/554313/8294/8359/8360 (Human)
100041230/319155/319156/319157/319158 (Mouse)
291152/295277/64627 (Rat)

SwissPro:

P62805 (Human)
P62806 (Mouse)
P62804 (Rat)

Immunogen
Information

Subcellular Location:

Nucleus. Chromosome.

Immunogen:

Synthetic peptide corresponding to the amino terminus of Histone H4 in which Lys20 is mono-methylated.

Specificity:

Histone H4(tri methyl K20) Monoclonal Antibody detects endogenous levels of Histone H4(tri methyl K20) protein.

Product images

Histone H4(mono methyl K20) Recombinant Mouse Monoclonal Antibody - 1

Fig : Western blot analysis of Histone H4(mono methyl K20) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06035, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell
Lane 2: C2C12 cell
Lane 3: EL-4-B5 cell
Lane 4: Mouse thymus

Predicted molecular weight:11 kDa
Observed molecular weight:15 kDa