Histone H4(mono methyl K20) Recombinant Mouse Monoclonal Antibody

货号：

AWA06035

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 11 kDa
Observed MW: 15 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.154mg/ml

Other Names:

dJ160A22.2; FO108; H4; H4-12; H4-16; H4-53; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4C1; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4f16; H4F2; H4F2iv; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; Hist1h4m; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; X04652; H4K20(ME1); H4K20me1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF 1:100-1:800

Immunogen
Information

Gene Name:

H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16

Protein Name:

Histone H4

Gene ID:

121504/554313/8294/8359/8360 (Human)
100041230/319155/319156/319157/319158 (Mouse)
291152/295277/64627 (Rat)

SwissPro:

P62805 (Human)
P62806 (Mouse)
P62804 (Rat)

Immunogen
Information

Subcellular Location:

Nucleus. Chromosome.

Immunogen:

Synthetic peptide corresponding to the amino terminus of Histone H4 in which Lys20 is mono-methylated.

Specificity:

Histone H4(tri methyl K20) Monoclonal Antibody detects endogenous levels of Histone H4(tri methyl K20) protein.

Product images
	Fig : Western blot analysis of Histone H4(mono methyl K20) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06035, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: C2C12 cell Lane 3: EL-4-B5 cell Lane 4: Mouse thymus Predicted molecular weight:11 kDa Observed molecular weight:15 kDa

	Fig: Immunocytochemistry analysis of Hela cells labeling Histone H4(mono methyl K20) with Mouse anti-Histone H4(mono methyl K20) antibody (AWA06035) at 1/150 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-Histone H4(mono methyl K20) antibody (AWA06035) at 1/150 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Mouse anti- Histone H4(mono methyl K20) antibody AWA06035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.