Histone H3(Acetyl Lys9/27) Recombinant Mouse Monoclonal Antibody

货号：

AWA06029

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat,Monkey

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat,Monkey

Molecular Wt:

Predicted MW: 15 kDa
Observed MW: 17 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

0.904mg/ml

Other Names:

H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Acetyl-Histone H3 (Lys9/27)

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF 1:25-1:200

Immunogen
Information

Gene Name:

H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12

Protein Name:

Histone H3.1

Gene ID:

8350/8351/8352/8353/8354 (Human)
319152/319153/360198/97908 (Mouse)
291159/679994/680498 (Rat)

SwissPro:

P68431 (Human)
P68433 (Mouse)
Q6LED0 (Rat)

Immunogen
Information

Subcellular Location:

Nucleus. Chromosome.

Immunogen:

The peptide from N-terminus of human Histone H3.1.

Specificity:

Histone H3(Acetyl Lys9/27) Monoclonal Antibody detects endogenous levels of Histone H3(Acetyl Lys9/27) protein.

Product images
	Fig : Western blot analysis of Histone H3(Acetyl Lys9/27) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06029, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse kidney Lane 2: Rat kidney Predicted molecular weight:15 kDa Observed molecular weight:17 kDa
	Fig : Western blot analysis of Histone H3(Acetyl Lys9/27) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06029, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: HL-60 cell Lane 3: COS7 cell Predicted molecular weight:15 kDa Observed molecular weight:17 kDa
	Fig: Immunocytochemistry analysis of Hela cells labeling Histone H3(Acetyl Lys9/27) with Mouse anti-Histone H3(Acetyl Lys9/27) antibody (AWA06029) at 1/25 dilution(Red). Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-Histone H3(Acetyl Lys9/27) antibody (AWA06029) at 1/25 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).