MFF Recombinant Mouse Monoclonal Antibody

货号：

AWA06026

应用：

WB,IHC-P,IF,FCM

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 38 kDa
Observed MW: 38 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.149mg/ml

Other Names:

C2orf33; mitochondrial fission factor; EMPF2; GL004; MFF

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500
IF 1:50-1:500
FCM 1:50-1:200

Immunogen
Information

Gene Name:

MFF

Protein Name:

Mitochondrial fission factor

Gene ID:

56947 (Human)
75734 (Mouse)
301563 (Rat)

SwissPro:

Q9GZY8 (Human)
Q6PCP5 (Mouse)
Q4KM98 (Rat)

Immunogen
Information

Subcellular Location:

Mitochondrion outer membrane. Peroxisome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

Immunogen:

Fusion protein amino acids 1-173 (cytoplasmic N-terminal exons 1, 2, 3 and 4) and 272-322 (cytoplasmic N-terminal exons 8 and most of 9) of mostly human MFF.

Specificity:

MFF Monoclonal Antibody detects endogenous levels of MFF protein.

Product images
	Fig : Western blot analysis of MFF on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06026, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse brain Lane 2: Rat brain Predicted molecular weight:38 kDa Observed molecular weight:38 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Mouse anti- MFF antibody (AWA06026) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06026) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of DU145 cells labeling MFF with Mouse anti-MFF antibody (AWA06026) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.5% Triton X-100 in PBS for 15 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-MFF antibody (AWA06026) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).