LGI1 Recombinant Mouse Monoclonal Antibody

货号：

AWA06024

应用：

WB,IHC-P,IF

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 64 kDa
Observed MW: 64 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1mg/ml

Other Names:

EPITEMPIN; Epitempin 1; EPT; ETL1; IB1099; ADLTE; ADPAEF; ADPEAF; LGI1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:500
IF 1:50-1:200

Immunogen
Information

Gene Name:

LGI1

Protein Name:

Leucine-rich glioma-inactivated protein 1

Gene ID:

9211 (Human)
56839 (Mouse)
252892 (Rat)

SwissPro:

O95970 (Human)
Q9JIA1 (Mouse)
Q8K4Y5 (Rat)

Subcellular Location:

Secreted. Synapse. Cytoplasm. Golgi apparatus. Endoplasmic reticulum.

Immunogen:

Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse Lgi1 (accession number Q9JIA1) produced recombinantly in E. Coli.

Specificity:

LGI1 Monoclonal Antibody detects endogenous levels of LGI1 protein.

Product images
	Fig : Western blot analysis of LGI1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06024, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U87-MG cell Lane 2: Rat brain Predicted molecular weight:64 kDa Observed molecular weight:64 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-hippocampus tissue with Mouse anti- LGI1 antibody (AWA06024) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06024) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.