Collagen Ⅱ Recombinant Mouse Monoclonal Antibody

货号：

AWA06023

应用：

WB,IHC-P

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 142 kDa
Observed MW: 142 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.124mg/ml

Other Names:

Alpha 1 type II collagen; ANFH; AOM; COL11A3; COL2A1; Col2α1; Collagen alpha 1(II) chain; Collagen Type II; collagen, type II; alpha 1; SEDC; STL1; Collagen Ⅱ

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000

Immunogen
Information

Gene Name:

COL2A1

Protein Name:

Collagen alpha-1(II) chain

Gene ID:

1280 (Human)
12824 (Mouse)
25412 (Rat)

SwissPro:

P02458 (Human)
P28481 (Mouse)
P05539 (Rat)

Immunogen
Information

Subcellular Location:

Secreted, extracellular space, extracellular matrix.

Immunogen:

Synthetic peptide with amino acids 551–564 of Collagen Ⅱ : MPGERRGAAGIAGPK.

Specificity:

Collagen Ⅱ Monoclonal Antibody detects endogenous levels of Collagen Ⅱ protein.

Product images
	Fig : Western blot analysis of Collagen II on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06023, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat articular cartilage Predicted molecular weight:142 kDa Observed molecular weight:142 kDa

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-articulatio genus tissue with Mouse anti- Collagen Ⅱ antibody (AWA06023) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06023) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.