Collagen Ⅱ Recombinant Mouse Monoclonal Antibody

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货号:
AWA06023
应用:
WB,IHC-P
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    Predicted MW: 142 kDa
    Observed MW: 142 kDa

    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1.724mg/ml

    Other Names:

    Alpha 1 type II collagen; ANFH; AOM; COL11A3; COL2A1; Col2α1; Collagen alpha 1(II) chain; Collagen Type II; collagen, type II; alpha 1; SEDC; STL1; Collagen II

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:500

    Immunogen
    Information

    Gene Name:

    COL2A1

    Protein Name:

    Collagen alpha-1(II) chain

    Gene ID:

    1280 (Human)
    12824 (Mouse)
    25412 (Rat)

    SwissPro:

    P02458 (Human)
    P28481 (Mouse)
    P05539 (Rat)

    Subcellular Location:

    Secreted, extracellular space, extracellular matrix.

    Immunogen:

    Synthetic peptide with amino acids 551–564 of Collagen Ⅱ : MPGERRGAAGIAGPK.

    Specificity:

    Collagen Ⅱ Monoclonal Antibody detects endogenous levels of Collagen Ⅱ protein.

    Product images
    Collagen Ⅱ Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of Collagen II on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06023, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Rat articular cartilage

    Predicted molecular weight:142 kDa
    Observed molecular weight:142 kDa
    Exposure time:90s

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