Calbindin Recombinant Mouse Monoclonal Antibody

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货号:
AWA06020
应用:
WB,IHC-P,FCM
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    Predicted MW: 30 kDa
    Observed MW: 30 kDa

    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml

    Other Names:

    CAB27; CALB; CALB1; CALB 1; calbindin 1 28kDa; Calbindin 1 28kDa; Calbindin D28; Calbindin-D28k; D28K; D 28K; D-28K; avian-type; RTVL H protein; Vitamin D dependent calcium binding protein; Calbindin

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:500-1:5000
    IHC-P 1:100-1:500
    FCM 1:50-1:200

    Immunogen
    Information

    Gene Name:

    CALB1

    Protein Name:

    Calbindin

    Gene ID:

    793 (Human)
    12307 (Mouse)
    83839 (Rat)

    SwissPro:

    P05937 (Human)
    P12658 (Mouse)
    P07171 (Rat)

    Subcellular Location:

    Axon. calyx of Held. cytosol. dendrite. dendritic spine. extracellular exosome. GABA-ergic synapse. glutamatergic synapse. hippocampal mossy fiber to CA3 synapse. neuronal cell body. nucleus. postsynaptic cytosol. presynaptic cytosol. synapse. terminal bouton.

    Immunogen:

    Fusion protein amino acids 1-261 (full-length) of human Calbindin.

    Specificity:

    Calbindin Monoclonal Antibody detects endogenous levels of Calbindin protein.

    Product images
    Calbindin Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of Calbindin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06020, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse kidney
    Lane 2: Rat kidney

    Predicted molecular weight:30 kDa
    Observed molecular weight:30 kDa
    Exposure time:90s
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