GLP1R Recombinant Mouse Monoclonal Antibody

货号：

AWA06019

应用：

WB,IF

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

Predicted MW: 53 kDa
Observed MW: 53 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1.172mg/ml

Other Names:

GLP-1-R; GLP-1R; GLP-1 receptor; GLP 1R; GLP 1 receptor; GLP-1; GLP1R

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IF 1:50-1:500

Immunogen
Information

Gene Name:

GLP1R

Protein Name:

Glucagon-like peptide 1 receptor

Gene ID:

2740 (Human)
14652 (Mouse)
25051 (Rat)

SwissPro:

P43220 (Human)
O35659 (Mouse)
P32301 (Rat)

Immunogen
Information

Subcellular Location:

Cell membrane.

Immunogen:

Synthesized peptide derived from human GLP1R. AA range: 24-145.

Specificity:

GLP1R Monoclonal Antibody detects endogenous levels of GLP1R protein.

Product images
	Fig : Western blot analysis of GLP1R on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06019, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse stomach Predicted molecular weight:53 kDa Observed molecular weight:53 kDa

	Fig: Immunocytochemistry analysis of HSC-T6 cells labeling GLP1R with Mouse anti-GLP1R antibody（AWA06019）at 1/50 dilution(Red). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-GLP1R antibody （AWA06019）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, AWS0004) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).