CD16/32 Recombinant Mouse Monoclonal Antibody

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货号:
AWA06009
应用:
WB,FCM
反应性:
Mouse
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Mouse

    Molecular Wt:

    29 kDa

    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml

    Other Names:

    CD16a; CD16b; CD32; CD32A; CD32B; CDw32; Fc fragment of IgG low affinity IIa receptor; Fc fragment of IgG low affinity IIb receptor; Fc fragment of IgG low affinity IIIa recepto; Fc fragment of IgG low affinity IIIb receptor; Fc gamma receptor III A; Fc gamma receptor IIIb; Fc gamma RIIa; Fc gamma RIIb; Fc gamma RIII beta; Fc-gamma RII-a; Fc-gamma-RIIa antibody FCG2A; FcGR; FCGR2A; FCGR2B; FCGR3; FCGR3A; FCGR3B; FcRII-a; FcRIII; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:2000
    FCM 1:50-1:200

    Immunogen
    Information

    Gene Name:

    FCGR3A

    Protein Name:

    Low affinity immunoglobulin gamma Fc region receptor III-A

    Gene ID:

    2214 (Human)
    14130 (Mouse)

    SwissPro:

    P08637 (Human)
    P08508 (Mouse)

    Subcellular Location:

    Cell membrane. Secreted.

    Immunogen:

    Mouse BALB/c Macrophage J774.

    Specificity:

    CD16/32 Monoclonal Antibody detects endogenous levels of CD16/32 protein.

    Product images
    CD16/32 Recombinant Mouse Monoclonal Antibody - 1 Fig:Flow cytometric analysis of J774A.1 cells labeling CD16/32.
    Overlay histogram showing J744A.1 cells stained with CD16/32 (green line). The cell were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA06009, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody (AWS0003a) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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