MMP9 Recombinant Mouse Monoclonal Antibody

货号：

AWA06008

应用：

WB,IHC-P,FCM

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 78 kDa Observed MW: 78 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1mg/ml

Other Names:

MMP9; CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:2000
IHC-P 1:100-1:1000
FCM 1:50-1:100

Immunogen
Information

Gene Name:

MMP9

Protein Name:

Matrix metalloproteinase-9

Gene ID:

4318 (Human)
17395 (Mouse)
81687 (Rat)

SwissPro:

P14780 (Human)
P41245 (Mouse)
P50282 (Rat)

Subcellular Location:

Secreted, extracellular space, extracellular matrix.

Immunogen:

Fusion protein amino acids 1-708 (full length) of rat MMP9.

Specificity:

MMP9 Monoclonal Antibody detects endogenous levels of MMP9 protein

Product images
	Fig : Western blot analysis of MMP9 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06008, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen Lane 2: Mouse lung Lane 3: Rat lung Predicted molecular weight:79 kDa Observed molecular weight:79 kDa Exposure time：90s
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Mouse anti-MMP9 antibody (AWA06008) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA06008) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.