E-Cadherin Recombinant Mouse Monoclonal Antibody

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货号:
AWA06006
应用:
WB,IP,ELISA
反应性:
Human,Dog
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human,Dog

    Molecular Wt:

    Predicted MW: 97 kDa Observed MW: 130 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml

     

    Other Names:

    CDH1; CD324; CAM 120/80; Cadherin-1; Cadherin 1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; E-Cadherin

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:500-1:2000
    IP 1-2ug/sample
    ELISA 1:20000

    Immunogen
    Information

    Gene Name:

    CDH1

    Protein Name:

    Cadherin-1

     

    Gene ID:

    999 (Human)

    SwissPro:

    P12830 (Human)

     

    Subcellular Location:

    Cell junction, adherens junction. Cell membrane. Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm. Cell junction, desmosome.

     

    Immunogen:

    Recombinant protein within human E-Cadherin. AA range: 155-707.

     

    Specificity:

    E-Cadherin Monoclonal Antibody detects endogenous levels of E-Cadherin protein.


    Product images
    E-Cadherin Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of E-Cadherin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06006, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: MCF-7 cell
    Lane 2: HaCaT cell
    Lane 3: Caco-2 cell
    Lane 4: A431 cell

    Predicted molecular weight:97 kDa
    Observed molecular weight:130 kDa
    Exposure time:3min
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