Bax Recombinant Mouse Monoclonal Antibody

货号：

AWA06004

应用：

WB,FCM,ELISA

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 21 kDa
Observed MW: 21 kDa

Clonality:

Monoclonal

Isotype:

IgG1

Concentration:

1mg/ml

Other Names:

BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; Bax-protein; BAXA; BCL2 associated X protein; membrane isoform alpha

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-2000
FCM 1:50-1:200
ELISA 1:10000

Immunogen
Information

Gene Name:

BAX

Protein Name:

Apoptosis regulator BAX

Gene ID:

581 (Human)
12028 (Mouse)
24887 (Rat)

SwissPro:

Q07812 (Human)
Q07813 (Mouse)
Q63690 (Rat)

Subcellular Location:

Mitochondrion outer membrane. Cytoplasm. Nucleus.

Immunogen:

Synthesized peptide derived from human Bax. AA range: 12-24.

Specificity:

Bax Monoclonal Antibody detects endogenous levels of Bax protein.

Product images
	Fig : Western blot analysis of Bax on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06004, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: Raji cell Lane 3: Raw264.7 cell Lane 4: RSC96 cell Predicted molecular weight:21 kDa Observed molecular weight:21 kDa Exposure time：7min
	Fig：Flow cytometric analysis of RAW264.7 cells labeling Bax. Overlay histogram showing RAW264.7 cells stained with Bax (green line). The cells were fixed in 4% paraformaldehyde for 30 minutes at 37 ℃, permeabilized with 0.02% Triton X-100 in PBS for 30 minutes,and then stained with the primary antibody(AWA06005, 1µg/1x106 cells) for 30 min at 4°C. The secondary antibody used was an Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody (AWS0003a) at 1/500 dilution for 30 min at 4ºC. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).