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TAGLN Recombinant Mouse Monoclonal Antibody

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货号:
AWA04523
应用:
WB,IHC-P,IF-C
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    23 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    1.034mg/ml

     

    Other Names:

    SM22; SM22 alpha; SM22-alpha; SM22a; SM22alpha; SMCC; TAGLN1; transgelin; transgelin/SM22; Smooth muscle protein 22-alpha; 22 kDa actin-binding protein; Protein WS3-10; WS3-10; TAGLN

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:10000
    IHC-P 1:100-1:1000
    IF-C 1:100-1:800

    Immunogen
    Information

    Gene Name:

    TAGLN

    Protein Name:

    Transgelin

     

    Gene ID:

    6876 (Human)  
    21345 (Mouse)  
    25123 (Rat)

    SwissPro:

    Q01995 (Human)  
    P37804 (Mouse)  
    P31232 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Cytoplasm.

     

    Immunogen:

    Synthetic peptide wihtin C-terminus of human Transgelin/TAGLN protein.

     

    Specificity:

    TAGLN Monoclonal Antibody detects endogenous levels of TAGLN protein.


    Product images
    TAGLN Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of TAGLN on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA04523, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse stomach
    Lane 2: Human colonic tissue
    Predicted molecular weight:23 kDa
    Observed molecular weight:23 kDa
    TAGLN Recombinant Mouse Monoclonal Antibody - 2 Fig : Western blot analysis of TAGLN on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA04523, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: CACO-2 cell
    Lane 2: AGS cell
    Lane 3: SK-N-SH cell
    Lane 4: Hela cell
    Lane 5: A2780 cell
    Lane 6: PC12 cell
    Lane 7: SAOS-2 cell
    Predicted molecular weight:23 kDa
    Observed molecular weight:23 kDa

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