CSPG4/NG2 Recombinant Mouse Monoclonal Antibody

货号：

AWA03133

应用：

WB,IHC-P,IF-T,FCM,ELISA

反应性：

Human,Mouse

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse

Molecular Wt:

251 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

CSPG4; HMW MAA; MCSP; MCSPG; MEL CSPG; MSK16; Neuron-glia 2; NG2; CSPG4/NG2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IF-T 1:100-1:1000
FCM 1:50-1:200
ELISA 1:20000

Immunogen
Information

Gene Name:

CSPG4

Protein Name:

Chondroitin sulfate proteoglycan 4

Gene ID:

1464 (Human)
121021 (Mouse)

SwissPro:

Q6UVK1 (Human)
Q8VHY0 (Mouse)

Immunogen
Information

Subcellular Location:

Cell membrane. Apical cell membrane. Cell projection, lamellipodium membrane. Cell surface.

Immunogen:

Recombinant protein within human CSPG4.

Specificity:

CSPG4/NG2 Monoclonal Antibody detects endogenous levels of CSPG4/NG2 protein.

Product images
	Fig : Western blot analysis of CSPG4/NG2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA03133, 1/3000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SK-MEL-28 cell Lane 2: OVCAR8 cell Lane 3: SIHa cell Predicted molecular weight:251 kDa Observed molecular weight:300 kDa

	Fig : Western blot analysis of CSPG4/NG2 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA03133, 1/3000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SCC25 cell Lane 2: A204 cell Lane 3: MDA-MB-231 cell Lane 4: HT1080 cell Lane 5: RD cell Predicted molecular weight:251 kDa Observed molecular weight:300 kDa