MUC2 Recombinant Mouse Monoclonal Antibody

货号：

AWA02739

应用：

IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse

Molecular Wt:

540 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

MLP; SMUC; MUC-2; MUC 2; Muc2; MUC2; Intestinal mucin 2; Mucin 2; Mucin 2 intestinal

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

IHC-P 1:50-1:200
IF-C 1:50-1:500
IF-T 1:100-1:500
FCM 1:50-1:200

Immunogen
Information

Gene Name:

MUC2

Protein Name:

Mucin-2

Gene ID:

4583 (Human)
17831 (Mouse)

SwissPro:

Q02817 (Human)
Q80Z19 (Mouse)

Immunogen
Information

Subcellular Location:

Secreted.

Immunogen:

Synthetic peptide within human MUC2. AA range: 5100-5149.

Specificity:

MUC2 Monoclonal Antibody detects endogenous levels of MUC2 protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Mouse anti- MUC2 antibody (AWA02739) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02739) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig: Immunocytochemistry analysis of HT-29 cells labeling MUC2 with Mouse anti- MUC2 antibody（AWA02739）at 1/50 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti- MUC2 antibody （AWA02739）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).