MUC2 Recombinant Mouse Monoclonal Antibody [46C05]

货号：

AWA02739

应用：

IHC-P,IF-C,mIHC(TSA)

反应性：

Human,Mouse,Rat

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Human, Mouse, Rat

Molecular Wt:

551 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

0.930mg/ml

Other Names:

MLP; SMUC; Intestinal mucin 2; Intestinal mucin-2; Mucin-2; Mucin 2; Mucin 2 intestinal; MUC-2; MUC 2; Muc2; MUC2

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

IHC-P 1:100-1:1000
IF-C 1:100-1:800

mIHC(TSA) 1:200-1:800

Immunogen
Information

Gene Name:

MUC2

Protein Name:

Mucin-2

Gene ID:

4583 (Human)

SwissPro:

Q02 817 (Human)
Q80Z19 (Mouse)
Q62635 (Rat)

Immunogen
Information

Subcellular Location:

Secreted.

Immunogen:

Synthetic peptide within human MUC2.

Specificity:

MUC2 Monoclonal Antibody detects endogenous levels of MUC2 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of Rat-ileum tissue (Formalin/PFA-fixed paraffin-embedded sections). with Mouse anti-MUC2 antibody (AWA02739) at 1/400 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02739) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.

	Fig: Immunocytochemistry analysis of HT-29 cells labeling MUC2 with Mouse anti- MUC2 antibody（AWA02739）at 1/100 dilution(Green ). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti- MUC2 antibody （AWA02739）at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-small intestine tissue with Mouse anti- MUC2 antibody (AWA02739) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02739) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig : Immunohistochemical analysis of paraffin-embedded Rat-rectum tissue with Mouse anti-MUC2 antibody (AWA02739) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA02739) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.