ds DNA Recombinant Mouse Monoclonal Antibody [47C01]

货号：

AWA02664

应用：

IHC-P,IF-C

反应性：

Speciesindependent

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Speciesindependent

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

0.555mg/ml

Other Names:

Double stranded DNA; Double stranded deoxyribonucleic acid; dsDNA; ds DNA

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

IHC-P 1:50-1:200
IF-C 1:50-1:200

Immunogen
Information

Specificity:

ds DNA Monoclonal Antibody detects endogenous levels of ds DNA protein.

Product images
	Fig: Immunocytochemistry analysis of Hela cells labeling ds DNA with Mouse anti-ds DNA antibody（AWA02664）at 1/200 dilution（Green）. Cells were fixed in 100% Methanol（-20℃ precool） for 20 minutes, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Mouse anti-ds DNA antibody （AWA02664） at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L （iFluor™ 488, AWS0003） was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI（AWC0291）.

	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-skin tissue with Mouse anti- ds DNA antibody （AWA02664） at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer （pH 6.0） for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody （AWA02664） at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system（ABIOWELL, AWI0629）. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Dot blot analysis of ds DNA using AWA02664 at 1:1000 followed by a Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated (AWS0001) at 1:5000 dilution. Lane 1: Total DNA was isolated from Hela cell Blocking and diluting buffer and concentration: 5% NF-Milk/TBST