CD8 alpha Recombinant Mouse Monoclonal Antibody

货号：

AWA01296

应用：

WB,IHC-P,IHC-F,IP,FCM

反应性：

Mouse

来源：

Mouse

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Mouse

Reactivity:

Mouse

Molecular Wt:

26 kDa

Clonality:

Monoclonal

Isotype:

IgG2a

Concentration:

1mg/ml

Other Names:

CD8 antigen; CD8A molecule; CD8 antigen alpha polypeptide; Cd8a; CD8; Leu2; MAL; p32; Ly 2; Ly-2; Ly 35; Ly-35; Ly B; Ly-B; Lyt 2; Lyt-2; CD8A; CD8 alpha

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:1000
IHC-F 1:100-1:1000
IP 0.5-4.0 ug for 0.5-3.0 mg of total protein lysate.
FCM 1:50-1:200

Immunogen
Information

Gene Name:

CD8A

Protein Name:

T-cell surface glycoprotein CD8 alpha chain

Gene ID:

12525 (Mouse)

SwissPro:

P01731 (Mouse)

Immunogen
Information

Subcellular Location:

Cell membrane. Secreted.

Immunogen:

Recombinant protein within human CD8 alpha.

Specificity:

CD8 alpha Monoclonal Antibody detects endogenous levels of CD8 alpha protein.

Product images
	Fig : Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA01296, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse thymus Predicted molecular weight:26 kDa Observed molecular weight:30~40 kDa

	Fig : Western blot analysis of CD8 alpha on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA01296, 1/2000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen Predicted molecular weight:26 kDa Observed molecular weight:30~40 kDa