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GSK3β(Phospho Ser9) Recombinant Mouse Monoclonal Antibody [47B05]

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货号:
AWA00709
应用:
WB,IF-C
反应性:
Human,Mouse,Rat
来源:
Mouse
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Human, Mouse, Rat

    Molecular Wt:

    47 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG2a

    Concentration:

    1.092mg/ml

     

    Other Names:

    GSK 3 beta; GSK3 beta; GSK3A B; GSK3B; GSK-3B; GSK3B 216P; gsk3beta; GSK-3beta; GSK-3 beta; GSK3β; Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK3beta isoform; GSK3β; GSK3β(Phospho Ser9)

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:5000
    IF-C 1:100-1:800

    Immunogen
    Information

    Gene Name:

    GSK3B

    Protein Name:

    Glycogen synthase kinase-3 beta

     

    Gene ID:

    2932 (Human)    
    56637 (Mouse)    
    84027 (Rat)

    SwissPro:

    P49841 (Human)    
    Q9WV60 (Mouse)    
    P18266 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Cytoplasm. Nucleus. Cell membrane.

     

    Immunogen:

    Synthetic phospho-peptide corresponding to residues surrounding Ser9 of human GSK3β. AA range: 1-50.

     

    Specificity:

    Phospho GSK3β (Ser9) Monoclonal Antibody detects endogenous levels of Phospho GSK3β (Ser9) protein.


    Product images
    GSK3β(Phospho Ser9) Recombinant Mouse Monoclonal Antibody [47B05] - 1 Fig : Western blot analysis of GSK3β(Phospho Ser9) on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA00709, 1/8000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: PC-3 cell
    Lane 3: NIH3T3 cell
    Lane 4: AML12 cell
    Lane 5: C6 cell
    Lane 6: HSC-T6 cell
    Lane 7: PC12 cell
    Predicted molecular weight:47 kDa
    Observed molecular weight:47 kDa

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