α-SMA Rabbit Polyclonal Antibody

货号：

AWA46841

应用：

WB,IHC-P,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

42 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:500-1:2000

IHC-P 1:100-1:300

ELISA 1:20000

Immunogen

Information

Gene Name：

ACTA2

Protein Name：

Actin, aortic smooth muscle

Gene ID：

59 (Human)

11475 (Mouse)

81633 (Rat)

SwissPro：

P62736 (Human)

P62737 (Mouse)

P62738 (Rat)

Subcellular Location：

Cytoplasm.

Immunogen：

Synthesized peptide derived from the N-terminal region of human α-SMA. AA range: 84-134.

Specificity:

α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue with Rabbit anti-αSMA (AWA46841) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46841) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-large intestine tissue with Rabbit anti-αSMA (AWA46841) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA46841) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Western blot analysis of α-SMA on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA46841, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell Lane 2: Jurkat cell Lane 3: RBL-2H3 cell Lane 4: RAW264.7 cell Lane 5: EL-4-B5 cell Lane 6: Hela cell Lane 7: Ishikawa cell Lane 8: NIH/3T3 cell Lane 9: L929 cell Lane 10: HEK293 cell Lane 11: 786-O cell Predicted molecular weight: 42kDa Observed molecular weight: 42kDa Exposure time: 7 seconds
	Fig : Western blot analysis of α-SMA on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody ( AWA46841, 1/1000) was used in PBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 12: NRK-49F cell Lane 13: PC12 cell Lane 14: A2058 cell Lane 15: C2C12 cell Lane 16: N2A cell Lane 17: HT22 cell Lane 18: SH-SY5Y cell Lane 19: U87-MG cell Lane 20: A549 cell Lane 21: BEAS-2B cell Lane 22: LLC cell Predicted molecular weight: 42kDa Observed molecular weight: 42kDa Exposure time: 7 seconds