COX IV Rabbit Polyclonal Antibody

货号：

AWA61720

应用：

WB,IHC-P,IF,IP,ELISA

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 20 kDa
Observed MW: 17 kDa

Clonality:

Polyclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

COX4I1; COX4; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:1000-1:5000
IHC-P 1:100-1:500
IF 1:50-1:200
IP 1:100-1:300
ELISA 1:20000

Immunogen
Information

Gene Name:

COX4I1

Protein Name:

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Gene ID:

1327 (Human)
12857 (Mouse)
29445 (Rat)

SwissPro:

P13073 (Human)
P19783 (Mouse)
P10888 (Rat)

Subcellular Location:

Mitochondrion inner membrane.

Immunogen:

Recombinant protein within human COX IV. AA range: 23-98.

Specificity:

COX IV Polyclonal Antibody detects endogenous levels of COX IV protein.

Product images
	Fig : Immunohistochemical analysis of paraffin-embedded Mouse-Kidney tissue with Rabbit anti-COX Ⅳ antibody (AWA61720) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA61720) at 1/200 dilution for 2 hour at 37℃or overnignt at 4℃. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig: Immunocytochemistry analysis of A431 cells labeling COX IV with Rabbit anti-COX IV antibody (AWA61720) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti- COX IV antibody (AWA61720) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of COX IV on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA61720, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 5637 cell Lane 2: U87-MG cell Lane 3: SK-BR-3 cell Lane 4: HELA cell Lane 5: THP-1 cell Lane 6: HEPG2 cell Lane 7: A549 cell Lane 8: 4T1 cell Lane 9: GL261 cell Predicted molecular weight:20 kDa Observed molecular weight:17 kDa Exposure time：45seconds