Lamin A/C Rabbit Polyclonal Antibody
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- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
Product Details | Host Species: Rabbit | Reactivity: Human,Mouse,Rat,Monkey,Pig | Molecular Wt: 65,74 kDa | |
Clonality: Polyclonal | Isotype: IgG | Concentration: 1mg/ml | ||
Other Names: LMNA; LMN1; Prelamin-A/C; 70 kDa lamin; CDDC; FPL; IDC; LFP; EMD2; FPLD; HGPS; LDP1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Lamin A/C | ||||
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. | ||||
Purification: Affinity-chromatography | ||||
Storage: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. | ||||
Applications | WB 1:500-1:2000 | |||
Immunogen Information | Gene Name: LMNA | Protein Name: Prelamin-A/C | ||
Gene ID: 4000 (Human) | SwissPro: P02545 (Human) | |||
Subcellular Location: Nucleus lamina. Nucleus envelope. Nucleus, nucleoplasm. Nucleus matrix. Nucleus speckle. | ||||
Immunogen: The antiserum was produced against synthesized peptide derived from human Lamin A/C. AA range: 361-410. | ||||
Specificity: Lamin A/C Polyclonal Antibody detects endogenous levels of Lamin A/C protein. | ||||
Product images | |
Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Immunohistochemical analysis of paraffin-embedded Rat-small intestine tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig : Western blot analysis of Lamin A/C on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42385, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell Lane 2: COS7 cell Lane 3: Hela cell Lane 4: HUVEC cell Lane 5: A375 cell Lane 6: NIH3T3 cell Exposure time: 7 seconds Predicted band size: 65,74 kDa Observed band size: 65,74 kDa |
-
-
- 20μL
- ¥620
- 1-3个工作日
-
- 50μL
- ¥1250
- 1-3个工作日
-
- 100μL
- ¥2200
- 1-3个工作日
-
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