Lamin A/C Rabbit Polyclonal Antibody

货号:
AWA42385
应用:
WB,IHC,IF,ELISA
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 有库存
  • 50μL 
  • ¥1250
  • 有库存
  • 100μL 
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    74+65 kDa


    Clonality:

    Polyclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    LMNA; LMN1; Prelamin-A/C


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC 1:100-1:300

    IF 1:200-1:1000

    ELISA 1:20000

    Not yet tested in other applications.

     



    Immunogen

    Information

    Gene Name:

    LMNA

    Protein Name:

    Prelamin-A/C


    Gene ID:

    4000 (Human)     

    16905 (Mouse)     

    60374 (Rat)

    SwissPro

    P02545 (Human)     

    P48678 (Mouse)     

    P48679 (Rat)

     


    Subcellular Location:

    Nucleus. Nucleus envelope. Nucleus lamina. Nucleus, nucleoplasm. Nucleus matrix.


    Immunogen

    The antiserum was produced against synthesized peptide derived from human Lamin A/C. AA range:361-410.

     


    Specificity:

    Lamin A/C Polyclonal Antibody detects endogenous levels of Lamin A/C protein.




    Product images
    Lamin A/C Rabbit Polyclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded Rat-cerebellum tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Lamin A/C Rabbit Polyclonal Antibody - 2 Fig : Immunohistochemical analysis of paraffin-embedded Rat-small intestine tissue with Rabbit anti-Lamin A-C (AWA42385) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA42385) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Lamin A/C Rabbit Polyclonal Antibody - 3 Fig : Western blot analysis of Lamin A/C on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA42385, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell
    Lane 2: COS7 cell
    Lane 3: Hela cell
    Lane 4: HUVEC cell
    Lane 5: A375 cell
    Lane 6: NIH3T3 cell


    Exposure time: 7 seconds
    Predicted band size: 65/74 kDa
    Observed band size: 65/74 kDa
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