MFF Recombinant Rabbit Monoclonal Antibody

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货号:
AWA22017
应用:
WB,IHC-P,IF,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    Predicted MW: 38 kDa
    Observed MW: 38 kDa

     

    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    0.841mg/ml

     

    Other Names:

    C2orf33; mitochondrial fission factor; EMPF2; GL004; MFF

     

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

     

    Purification:

    Affinity-chromatography

     

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:500
    IF 1:50-1:200
    FCM 1:50-1:100

    Immunogen
    Information

    Gene Name:

    MFF

    Protein Name:

    Mitochondrial fission factor

     

    Gene ID:

    56947 (Human)
    75734 (Mouse)
    301563 (Rat)

    SwissPro:

    Q9GZY8 (Human)
    Q6PCP5 (Mouse)
    Q4KM98 (Rat)

    Immunogen
    Information

    Subcellular Location:

    Mitochondrion outer membrane. Peroxisome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle.

     

    Immunogen:

    Fusion protein amino acids 1-173 (cytoplasmic N-terminal exons 1, 2, 3 and 4) and 272-322 (cytoplasmic N-terminal exons 8 and most of 9) of mostly human MFF.

     

    Specificity:

    MFF Monoclonal Antibody detects endogenous levels of MFF protein.


    Product images
    MFF Recombinant Rabbit Monoclonal Antibody - 1 Fig : Western blot analysis of MFF on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22017, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Hela cell
    Lane 2: SIHa cell

    Predicted molecular weight:38 kDa
    Observed molecular weight:38 kDa
    MFF Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of MFF on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA22017, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: DU145 cell
    Lane 2: Mouse brain

    Predicted molecular weight:38 kDa
    Observed molecular weight:38 kDa

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