Caspase-8 Recombinant Rabbit Monoclonal Antibody_长沙艾碧维生物科技有限公司

货号：

AWA12688

应用：

WB,IF-C,IF-T

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ￥620 1-3个工作日
50μL ￥1250 1-3个工作日
100μL ￥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

55 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1 mg/ml

Other Names:

CAP4; MACH; MCH5; FLICE; ALPS2B; Caspase8; Caspase 8; Casp-8; CASP-8; CED 3; CASP8; FLJ17672; MGC78473; Apoptotic cysteine protease; Caspase-8 subunit p10; Apoptotic protease Mch-5; Apoptotic protease Mch5; FADD Like ICE; FADD-like ICE

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

-20°C,1 year

Applications

WB 1:1000-1:5000

IF-C 1:50-1:200

IF-T 1:50-1:200

Immunogen

Information

Gene Name：

CASP8

Protein Name：

Caspase-8

Gene ID：

841 (Human)

12370 (Mouse)

64044 (Rat)

SwissPro：

Q14790 (Human)

O89110 (Mouse)

Q9JHX4 (Rat)

Subcellular Location：

Cytoplasm. Nucleus. Cell projection, lamellipodium.

Immunogen：

Synthetic peptide within Human Caspase-8. AA range: 207-256.

Specificity:

Caspase-8 Monoclonal Antibody detects endogenous levels of Caspase-8 protein.

Product images
	Fig: Fluorescence immunohistochemical analysis of mouse-brain tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of mouse-liver tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Fluorescence immunohistochemical analysis of rat-brain tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig: Immunocytochemistry analysis of Hela cells labeling Caspase 8 with Rabbit anti-Caspase 8 antibody (AWA12688 )at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Caspase 8 antibody (AWA12688) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig : Western blot analysis of caspase 8 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12688, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 4T1 cell Lane 2: HELA cell Lane 3: NPK-49F cell Lane 4: RBL-2H3 cell Lane 5: K562 cell Lane 6: Jurkat cell Lane 7: N-2a cell Lane 8: NIH3T3 cell Lane 9: HEK-293 cell Lane 10: HEPA1-6 cell Predicted band size: 55 kDa Observed band size: 55 kDa (pro-caspase 8),40kd( pro-caspase8 p18),18kd( p18)
	Fig : Western blot analysis of Caspase-8 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12688, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell Lane 2: K562 cell Lane 3: HELA cell Predicted band size: 55 kDa Observed band size: 55 kDa