Caspase-8 Recombinant Rabbit Monoclonal Antibody

货号:
AWA12688
应用:
WB,IHC-P,IF-C,IF-T
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL 
  • ¥620
  • 1-3个工作日
  • 50μL 
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    55 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    CAP4; MACH; MCH5; FLICE; ALPS2B; Caspase8; Caspase 8; Casp-8; CASP-8; CED 3; CASP8; CASP8_HUMAN; FLJ17672; MGC78473; Apoptotic cysteine protease; Caspase-8 subunit p10; Apoptotic protease Mch-5; Apoptotic protease Mch5; FADD Like ICE; FADD-like ICE

     


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:1000-1:5000

    IHC-P 1:50-1:200

    IF-C 1:50-1:200

    IF-T 1:50-1:200

     



    Immunogen

    Information

    Gene Name:

    CASP8

    Protein Name:

    Caspase-8


    Gene ID:

    841 (Human)

    12370 (Mouse)

    64044 (Rat)

     

    SwissPro:

    Q14790 (Human)     

    O89110 (Mouse)     

    Q9JHX4 (Rat)


    Subcellular Location

    Cytoplasm. Nucleus. Cell projection, lamellipodium.


    Immunogen:

    Synthetic peptide within Human Caspase-8. AA range: 207-256.


    Specificity:

    Caspase-8 Monoclonal Antibody detects endogenous levels of Caspase-8 protein.




    Product images
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Fluorescence immunohistochemical analysis of mouse-brain tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of mouse-liver tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 3 Fig: Fluorescence immunohistochemical analysis of rat-brain tissue (Formalin / PFA-fixed paraffin-embedded sections) with rabbit anti-Caspase 8 antibody ( AWA12688 ) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA12688 )at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 4 Fig: Immunocytochemistry analysis of Hela cells labeling Caspase 8 with Rabbit anti-Caspase 8 antibody (AWA12688 )at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-Caspase 8 antibody (AWA12688) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0003) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 5 Fig : Western blot analysis of caspase 8 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12688, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: 4T1 cell
    Lane 2: HELA cell
    Lane 3: NPK-49F cell
    Lane 4: RBL-2H3 cell
    Lane 5: K562 cell
    Lane 6: Jurkat cell
    Lane 7: N-2a cell
    Lane 8: NIH3T3 cell
    Lane 9: HEK-293 cell
    Lane 10: HEPA1-6 cell

    Predicted band size: 55 kDa
    Observed band size: 55 kDa (pro-caspase 8),40kd( pro-caspase8 p18),18kd( p18)
    Caspase-8 Recombinant Rabbit Monoclonal Antibody - 6 Fig : Western blot analysis of Caspase-8 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA12688, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell
    Lane 2: K562 cell
    Lane 3: HELA cell

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
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