SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody

货号：

AWA10515

应用：

WB,IHC-P,IF-C,IF-T,FCM

反应性：

Human,Mouse,Rat

来源：

Rabbit

规格 价格 库存
20μL ¥620 1-3个工作日
50μL ¥1250 1-3个工作日
100μL ¥2200 1-3个工作日

产品概述

Product Details

Host Species:

Rabbit

Reactivity:

Human,Mouse,Rat

Molecular Wt:

Predicted MW: 48 kDa Observed MW: 62 kDa

Clonality:

Monoclonal

Isotype:

IgG

Concentration:

1mg/ml

Other Names:

P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62

Formulation:

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Purification:

Affinity-chromatography

Storage:

Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

Applications

WB 1:500-1:5000
IHC-P 1:200-1:1000
IF-C 1:50-1:100
IF-T 1:100-1:500
FCM 1:1000

Immunogen
Information

Gene Name:

SQSTM1

Protein Name:

Sequestosome-1

Gene ID:

8878 (Human)
18412 (Mouse)
113894 (Rat)

SwissPro:

Q13501 (Human)
Q64337 (Mouse)
O08623 (Rat)

Subcellular Location:

Cytoplasmic vesicle, autophagosome. Preautophagosomal structure. Cytoplasm, cytosol. Nucleus, PML body. Late endosome. Lysosome. Nucleus. Endoplasmic reticulum. Cytoplasm, myofibril, sarcomere.

Immunogen:

Synthetic peptide within human SQSTM1/ p62. AA range: 400 to the C-terminus.

Specificity:

SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.

Product images
	Fig: Immunocytochemistry analysis of U-251MG cells labeling SQSTM1/p62 with rabbit anti-SQSTM1/p62 antibody (AWA10515) at 1/50 dilution(green). Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with rabbit anti-P62 antibody (AWA10515) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-rabbit IgG H&L (iFluor™ 488, AWS0005) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Immunocytochemistry analysis of Hela cells labeling SQSTM1/p62 with Rabbit anti-SQSTM1/p62 antibody (AWA10515) at 1/50 dilution(Green). Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SQSTM1/p62 antibody （AWA10515）at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
	Fig: Fluorescence immunohistochemical analysis of Rat-cerebral cortex tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-SQSTM1/p62 antibody (AWA10515) at 1/200 dilution. The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0688). The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10515) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
	Fig : Western blot analysis of SQSTM1/p62 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody (AWA10515, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SHZ-88 cell Lane 2: RBL-2H3 cell Lane 3: HeLa cell Lane 4: NRF-49F cell Lane 5: NIH3T3 cell Lane 6: HEK293 cell Lane 7: HepG2 cell Lane 8: HCT116 cell Predicted molecular weight: 48 kDa Observed molecular weight: 62 kDa
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-SQSTM1/P62 antibody (AWA10515) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-SQSTM1/P62 antibody (AWA10515) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H₂O₂ for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.