SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10515
应用:
WB,IHC-P,IF-C,IF-T,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL 
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

     

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    Predicted MW: 48 kDa

    Observed MW: 62 kDa

     


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1 mg/ml


    Other Names:

    P62; P62/SQSTM1; sequestosome 1; SQSTM1; Ubiquitin binding protein p62 


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:5000

    IHC-P 1:1000-1:2000

    IF-C 1:100

    IF-T 1:200-1:1000

    FCM 1:1000

     



    Immunogen

    Information

    Gene Name:

    SQSTM1

    Protein Name:

    Sequestosome-1


    Gene ID:

    8878 (Human)     

    18412 (Mouse)     

    113894 (Rat)

    SwissPro:

    Q13501 (Human)     

    Q64337 (Mouse)     

    O08623 (Rat)

     


    Subcellular Location:

    Cytoplasm, Endoplasmic reticulum, Endosome, Lysosome, Nucleus.


    Immunogen:

    Synthetic peptide within Human SQSTM1/ p62. AA range: 400 to the C-terminus.


    Specificity:

    SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.




    Product images
    SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody - 1 Fig: Immunocytochemistry analysis of Hela cells labeling SQSTM1/p62 with Rabbit anti-SQSTM1/p62 antibody (AWA10515) at 1/50 dilution(Green).

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.03% Triton X-100 in PBS for 30 minutes, and then blocked with 5% BSA for 60 minutes at 37 ℃. Cells were then incubated with Rabbit anti-SQSTM1/p62 antibody (AWA10515)at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, AWS0005c) was used as the secondary antibody at 1/200 dilution for 60 minutes at 37 ℃. Nuclear DNA was labelled in blue with DAPI(AWC0291).
    SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of Rat-cerebral cortex tissue (Formalin/PFA-fixed paraffin-embedded sections). with Rabbit anti-SQSTM1/p62 antibody (AWA10515) at 1/200 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10515) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (green). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Western blot analysis of SQSTM1/P62 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody ( AWA10515, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHZ-88 cell lysate
    Lane 2: RBL-2H3 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: NRF-49F cell lysate
    Lane 5: NIH3T3 cell lysate
    Lane 6: HEK293 cell lysate
    Lane 7: HepG2 cell lysate
    Lane 8: HCT116 cell lysate

    Predicted molecular weight: 48 kDa
    Observed molecular weight: 62 kDa
    SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue with Rabbit anti-SQSTM1/P62 antibody (AWA10515) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Recombinant Rabbit Monoclonal Antibody - 5 Fig : Immunohistochemical analysis of paraffin-embedded Rat-colon tissue with Rabbit anti-SQSTM1/P62 antibody (AWA10515) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( AWA10515 ) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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