Ki67 Recombinant Rabbit Monoclonal Antibody

货号:
AWA10320
应用:
WB,IHC-P,IHC-F,IF-C,IF-T,mIHC,FCM
反应性:
Human,Mouse,Rat
来源:
Rabbit
  • 20μL
  • ¥620
  • 1-3个工作日
  • 50μL
  • ¥1250
  • 1-3个工作日
  • 100μL
  • ¥2200
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Rabbit

    Reactivity:

    Human,Mouse,Rat

    Molecular Wt:

    359 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG

    Concentration:

    1mg/ml


    Other Names:

    Antigen KI 67; Antigen KI-67; Antigen Ki67; Ki 67; KI67; KIA; MKI67; MIB; MIB 1; Antigen identified by monoclonal antibody Ki 67; Antigen identified by monoclonal antibody Ki-67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.



    Applications

    WB 1:500-1:2000
    IHC-P 1:100-1:1000
    IHC-F 1:100-1:1000
    IF-C 1:50-1:200
    IF-T 1:100-1:500
    mIHC 1:100-1:1000
    FCM 1:500-1:1000



    Immunogen

    Information

    Gene Name:

    MKI67

    Protein Name:

    Proliferation marker protein Ki-67


    Gene ID:

    4288 (Human)  
    17345 (Mouse)  
    246042 (Rat)

    SwissPro:

    P46013 (Human)  
    E9PVX6 (Mouse)  
    Q5RJM0 (Rat)


    Subcellular Location:

    Chromosome. Nucleus. Nucleus, nucleolus.


    Immunogen:

    Synthetic peptide within human Ki67. AA range: 1040-1080.


    Specificity:

    Ki67 Monoclonal Antibody detects endogenous levels of Ki67 protein.




    Product images
    Ki67 Recombinant Rabbit Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded rat-spleen tissue with Rabbit anti-Ki67 (AWA10320) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Ki67 Recombinant Rabbit Monoclonal Antibody - 2 Fig : Western blot analysis of Ki67 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA10320, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (AWS0002) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SIHa cell
    Lane 2: EL-4-B5 cell

    Predicted molecular weight:359 kDa
    Observed molecular weight:359 kDa
    Exposure time:45 sec
    Ki67 Recombinant Rabbit Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded rat-testicle tissue with Rabbit anti-Ki67 (AWA10320) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Ki67 Recombinant Rabbit Monoclonal Antibody - 4 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-large intestine tissue with Rabbit anti-Ki67 antibody (AWA10320) at 1/200 dilution.
    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Ki67 Recombinant Rabbit Monoclonal Antibody - 5 Fig : Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue with Rabbit anti-KI67 (AWA10320) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    Ki67 Recombinant Rabbit Monoclonal Antibody - 6 Fig : Immunohistochemical analysis of paraffin-embedded Rat-heart tissue with Rabbit anti-KI67 (AWA10320) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA10320) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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