SQSTM1/p62 Mouse Monoclonal Antibody

货号:
AWA00001
应用:
WB,IHC,ELISA,FCM
反应性:
Human
来源:
Mouse
  • 20μL
  • ¥620
  • 有库存
  • 50μL
  • ¥1250
  • 有库存
  • 100μL
  • ¥2200
  • 有库存
  • 产品概述
  • Product Details

     

    Host Species:

    Mouse

    Reactivity:

    Human,Mouse,Rat

         Molecular Wt:

         48 kDa


    Clonality:

    Monoclonal

    Isotype:

    IgG1



    Other Names:

    p60; p62; A170; OSIL; PDB3; ZIP3; p62B


    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


    Purification:

    Affinity-chromatography


    Storage:

    -20°C,1 year



    Applications

     

    WB 1:500-1:2000

    IHC 1:200-1:1000

    ELISA 1:10000

    FCM 1:200-1:400

     



    Immunogen Information

    Gene Name:

    SQSTM1 ORCA OSIL

    Protein Name:

    SQSTM


    Gene ID:

    8878 (Human)     

    18412 (Mouse)     

    113894 (Rat)

    SwissPro:

    Q13501 (Human)    

    Q64337 (Mouse)     

    O08623 (Rat)

     


    Immunogen:

    Purified recombinant fragment of human SQSTM1 (AA: 232-356) expressed in E. Coli.


    Specificity:

    SQSTM1/p62 Monoclonal Antibody detects endogenous levels of SQSTM1/p62 protein.




    Product images
    SQSTM1/p62 Mouse Monoclonal Antibody - 1 Fig : Immunohistochemical analysis of paraffin-embedded mouse-brain tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 2 Fig: Fluorescence immunohistochemical analysis of mouse-colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 3 Fig : Immunohistochemical analysis of paraffin-embedded mouse-colon tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 4 Fig: Fluorescence immunohistochemical analysis of mouse-cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 5 Fig: Fluorescence immunohistochemical analysis of mouse-hippocampus tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 6 Fig : Immunohistochemical analysis of paraffin-embedded mouse-kidney tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 7 Fig : Immunohistochemical analysis of paraffin-embedded rat-brain tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 8 Fig: Fluorescence immunohistochemical analysis of RAT - colon tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 9 Fig : Immunohistochemical analysis of paraffin-embedded rat-colon tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 10 Fig: Fluorescence immunohistochemical analysis of RAT -cortex tissue (Formalin / PFA-fixed paraffin-embedded sections) with mouse anti-SQSTM1 antibody (AWA00001) at 1/100 dilution.

    The immunostaining was performed with the TSA Immuno-staining Kit (ABIOWELL, AWI0689). The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 60 minutes at 37℃, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system followed by a separate fluorescent tyramide signal amplification system (red). DAPI (blue, AWC0291) was used as a nuclear counter stain. Image acquisition was performed with Slide Scanner.
    SQSTM1/p62 Mouse Monoclonal Antibody - 11 Fig : Immunohistochemical analysis of paraffin-embedded rat-kidney tissue with mouse anti-SQSTM1 antibody ( AWA00001 ) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with  Sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 3% H2O2 for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (AWA00001) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system(ABIOWELL, AWI0629). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
    SQSTM1/p62 Mouse Monoclonal Antibody - 12 Fig : Western blot analysis of SQSTM1 on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 90 minutes at room temperature. The primary antibody ( AWA00001, 1/1000) was used in TBST at room temperature for 90 minutes. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: SHZ-88 cell lysate
    Lane 2: RBL-2H3 cell lysate
    Lane 3: NRF-49F cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: HEK293 cell lysate
    Lane 6: HepG2 cell lysate
    Lane 7: HCT116 cell lysate
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