FLAG tag Recombinant Mouse Monoclonal Antibody

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货号:
AWA06018
应用:
WB,IF-C,IP,FCM
反应性:
Speciesindependent
来源:
Mouse
  • 50μL
  • ¥550
  • 1-3个工作日
  • 100μL
  • ¥900
  • 1-3个工作日
  • 200μL
  • ¥1500
  • 1-3个工作日
  • 产品概述
  • Product Details

    Host Species:

    Mouse

    Reactivity:

    Species independent

    Clonality:

    Monoclonal

    Isotype:

    IgG1

    Concentration:

    1mg/ml

    Other Names:

    Flag tag; anti-DDDDK; anti- DYKDDDDK

    Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    Purification:

    Affinity-chromatography

    Storage:

    Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.

    Applications

    WB 1:1000-1:2000
    IF-C 1:50-1:200
    IP 1-2ug/sample
    FCM 1:100-1:200

    Immunogen
    Information

    Protein Name:

    Flag tag, anti-DDDDK, anti- DYKDDDDK

    Immunogen:

    Synthesized peptide of DYKDDDDK

    Specificity:

    This antibody recognizes amino acid sequence: N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

    Product images
    FLAG tag Recombinant Mouse Monoclonal Antibody - 1 Fig : Western blot analysis of FLAG tag on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (AWA06018,1/1000) was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (AWS0001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HEK293F cell
    Lane 2: HEK293F transfected with Flag-tag cell lysate

    Observed molecular weight: 39 kDa

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